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  1. polymerase chain reaction / PCR | Learn Science at Scitable

    polymerase chain reaction / PCR Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA.

  2. The Biotechnology Revolution: PCR and Cloning Expressed Genes

    Jun 27, 1970 · The cloning of expressed genes and the polymerase chain reaction (PCR), two biotechnological breakthroughs of the 1970s and 1980s, continue to play significant roles in science …

  3. Multiplex real-time RT-PCR method for the diagnosis of SARS ... - Nature

    Feb 18, 2022 · On the other hand, the reverse transcription polymerase chain reaction (RT-PCR) is the most common and accepted as the gold standard method for the viral detection 9.

  4. Scientists Can Make Copies of a Gene through PCR - Nature

    In most cases, the polymerase chain reaction, or PCR, is their method of choice for quickly generating a sufficient amount of identical genetic material for study and analysis.

  5. A brighter future with advances in PCR

    First developed more than four decades ago as a biological research technique to amplify nucleic acids, polymerase chain reaction (PCR) technology still has untapped potential, with many exciting ...

  6. PCR-based techniques - Latest research and news | Nature

    Feb 11, 2026 · PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR …

  7. Mirror-image polymerase chain reaction | Cell Discovery - Nature

    Oct 17, 2017 · We show that the d-polymerase is able to amplify a 120-bp l-DNA sequence coding for the Escherichia coli 5S ribosomal RNA gene rrfB by mirror-image polymerase chain reaction, and …

  8. Development of a novel strategy to reduce diagnostic errors in real ...

    Nov 8, 2024 · Molecular diagnostic methods, such as conventional and real-time polymerase chain reaction (PCR), allow prompt and accurate diagnosis of infectious diseases by identifying the nucleic …

  9. Reverse transcription-quantitative PCR (RT-qPCR) without the need for ...

    Jul 15, 2023 · The procedure illustrated in this paper represents a new method for transcriptome analysis by PCR (Polymerase Chain Reaction), which circumvents the need for elimination of …

  10. Reverse transcription polymerase chain reaction - Nature

    Nov 27, 2025 · Reverse transcription polymerase chain reaction (RTPCR) is a technique to detect or quantify RNA. It uses a reverse transcriptase enzyme to convert RNA to DNA, followed by PCR to …